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I NEW JUDD TRACT
1 KINGr and KALAKAUA AVE. I
I , , I'
OF DR. COME
(Continued from Pace 1)
frmii those hml inoculated man
limiili ci sill) cultures, without any
, thing resembling success. Tills
Oarly worli. however, taught in many
tlolall'j.,of tcrhnlquo; details that lip
licar to tiB to ho essential to hiic
cw. Wo can, however, roiiort at pres
ent that we have succeeded In grow.
Ing the bacillus of leprosy In mixed
culture four times rrom three rimes
of leprosy I rtrluckerliorr twice, Iloll
mann once and tho writer nine). Of
these four rultureH the nret one
was 'Unfortunately vontnmlnatod
Willi n -spore-bonror: It was tarried
(in to the. tenth Reiteration In mlxe I
v culliiror It was found that In thU
liilxoi! culture huiltipllratlnii was
rnpltZ. nfter tlio first few genera
tions wro passed.
This mixed culture, after helm;
strrlod Jo. the tenth Reiteration, wu
I temporarily laid asldo to make way
or tlioserom! rulture. which con
IhIiiciI tin spnru-bearer. It appeur-
. cd poselhlo tti curry on thfs-llmr
. culture In mixed form Indefinitely?
No ilouht It will bo found practical
Inter to Ecparato It out pure, but
i wo followed the line of least resist
ance niul concentrated our efforts on
the second culture
-1 Tho so'ond succosiful culture win
carried to tho third generation; It
Was found to contain only S. chol-
' orao, amoebae and H. leprae. I'rom
f Jlils third generation of the mixed
culturo tho writer 8iiccoeded7"U
.- means ulUlyjatlli;;, In, se.cur,lnjj a.jmrc
' culturo of tho bacillus of leprosy.
This pure culturo has been grown
'' Utreo generations In Its pure state,
In addition to tho three previous
, generations In which It grew In mix-
uil culturo. the culturo tube hero
, exhibited- being tho sixth generation
of tho bacillus on artificial media,
tho Instlpthren generations having
grown In puro culture.
Tho lepra bacilli u tho third and
fourth mlxpd oiltures that wo huo
hecurcd havo not boon isolated as
l:or eating, drinking and cooking
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realize that you
yet nor nio the prospects of thess
belnp, Isolated verv good, olio of
these being of extremely slow growth
and the other containing a spore
hearing bacillus as a contamination.
We have, however, n number if oth
er tubes Inoculated from other case
which are not yet teady lo be car
ried forward, hut some of these look
very promising. Kroni these latter
wo hopo lo secure additional strains
of the gncltll. We bollcve from our
experience thus far that with In
creasing knowledge of the details of
tho work, wo nun expect In future
to Isolate lepra bacilli with much
Icbs difficulty than we at Ilrst expe
rienced. Our experience nppoars to Indicate
that tho bclow-nicutloneil points npj
essential lo succors, but we recog
nize that our experience Is ns yet
too limited to state positively that
success can not bo met with It ono
should dlBregnrd these points:
I'lrst Tho kind of amoeba ono
employs Is probably a matter of In
difference, provided It Is nctlve
enough to .migrate a crow u l'otrl
dish be for o It encysts, othcrwlso Its
original syinhla'nts can not bo ro.
placed by tho -syniblant of choice.
Second Wo emplnjed II. coll, II.
typhi murium and S. cholorno us
symhlants; we were only successful
when tho latter organism wbb used,
-Third Wo found 'It Is essential to
clear tho salt agar media of Clcgg
with an egg. Tho egg, even after
coagulation and filtration. Imparts
'sulllclcnt nutriment to the media to
I sustain life of the nmoebao and
Kourtli our Incubators lowing
to the use of "honie-mudo" gas)
fllictuutcd between 34 C and 38 C,
i but these minor variations appear
to havo no unfavorable inilueuco on
Fifth The experience of ono of
us ('llrln-l(erhorf) working In tho
dry air of Honolulu was that tho
media tubes should hao an abun
dance of water of condensation In
them, and should bo cupped to uvold
evaporation. In tho experlcnco of
tho writer, working In tho moist air
of Kalawao, this was not found to
Sixth Wo only met with success
In our Inoculations from rather
eurly, untreated cases. Failure, re
sulted from our Into cases or curly
cusecs that hud been under Chaul
moogru oil for u considerable period.
This, however, may havo been only
' Sevonth -Wo only employed ro-Icently-ilcposltcd,
ules, or tho seru from such nodlucs,
In making our Inoculations. We be
lieve tho sera containing bacilli Is
to ho preferred to tho nodule It
self, a rich media of human tlssuo
appearing to cause, a lack of balance
between tho number of amoeba and
tho number of the syniblant spirilla
present, and thus mitigates against
Klghth Clcgg repeatedly Inocu
lated the salno tube on three suc
cessive, days; we did not find this
practical, so endeavoied to succeed
by a single Inoculation. We found,
however, thut such single Inocula
tions resulted In tho amoeba destroy
ing tho lepra bacilli by phagocyto
sis. To overcome this wo allowed
tho amoeba to first encyst, then
luo"uluted the tuba with tho leprous
would appear that ono
t to cither one or tho
must resort to either one or tho
other of these methods;, cither thnIlla nro wan(e) iarcular.. llloy
iiioB mu.i. i.e Mn.icue. u . success.vu mur(prorH nnrt ,t ; -Ani)mx ,llut
days to replace tho bacilli lost, or , , nr(J fflm w,lpro , ,hwo ,,,,
wo must, await a, stage of tho amoohu ' nt rcse,t
when they aro no longer cnpahlo ofi , m ,
phagootosls. ACCOIIDINO TO mhlcos tecelved
NMnth-lii this, the ninth point.1 ut tho ,,ort lho jul)aaCse liner Nippon
our experlcnco differs considerably Maru nnll Baid from Yokohnmu with
from that of Clegg. Thut gentle- ,eltnatloii as Honolulu und Sun Fran
mini reconiDiundcil tho oxuinlnatlon csc()- Tho vt,BS efl nlo Japanese
of the tubes about tho tenth day ,()lt ymieril.iv.
for the purposo of us'ortulnlng 'j
whether niiiltlplli'iitlnn hud occurred. THE niJI'AltTUHK of the Canadian
Thin wo did In tho etuli part of Aiisltnllan liner Mnkiira fmm Vlelo
oui woil. and bollovo i''Tt r.ur first iju wai loporicd joslcri'.ay. The fn
falmies woro largol) X ia to ills- p.el s enrouto to Australian imits by
turning the tubes at this time. Wotho way of Honolulu and Suva.
are missing one of the greatest opportunities to buy a lot in the
residence district ?v Drop in and get full particulars.
now do not examine a tubo for the
first three weeks and nre Inclined
to believe that It Is better to wait
for four weeks before disturbing tho
Tenth Multiplication Is Indicated
In tho original tube by a chango In
tho morphology of tho bacillus.
While tho morphology nt tills time
vnrles somewhat, tho form wo look
for ns Indicating multiplication Is
a shart, plump, ncld.fnst orgnnlsm,
occurring In chains; In other words,
this organism, when
It begins to
ttrepto-toc'o-bncllluB. Later It tends uo '"vlilcil equally between tho ml
lo. return to tho form noted In Us-110' w"lel wnB ioue tllc "" ua
fc. hut this Is also variable, it ' Tll vllluo ' ln ostute ''ow"
being very pleomorphic. , " tll(J 1'" accounts filed from
Control Wnrlr 1900 to 1907 was:
oi .:..n.i i.i .i, .i 'Jnmiary 18. 1900 t 71.019.01
oughly thtoughont. Our control
work has demonstrated the following
First Our ntnoobn, beforo having
leprous material added to them, did
not contain any nchl-fnst bacilli.
Second Tho cholera culturo wo
worked with was appal clitly puro
and certainly did not contain any
suit agar media with leprous mate
rial was not followed by a multipli
cation of tho lepra bacilli, when the
S. cholera and amoebae had) not been
Fourth Tho mere heating of lep-
Third Tho Inoculation of tho
rous material lo GO C for 30 min
utes t method employed in obtaining
a puro culturo) did not render tho
bacillus capable of growing on artl
Fifth Tho bacillus of tuberculo
sis can bo excluded on tho iiipldlty
of growth of the puro culture wo
have, as well as by the appearance
of such growth. We Bhall, however,
oxclude this technical point by ani
mal Inoculation at an early date.
Other nccldentnl acid-last organ
isms have not been technically ex
eluded, hut that three lepers should,
by accident, nil have these relative.
1) raro k.),;uitUini on their bkltid
seems too Improbable to consider.
Wo hopo to bo ablo to give the
results of nnlmul Inoculations and
cultural peculiarities at some futuro
The Impnrtnnco of this advance in
tliQ Investigation of leprosy is too
apparent to medical men to require
Donald H. Curric, Passed Assistant
Surgeon, U. S. P. H. & M. H. S ,
Director U S. Leprosy Investi
That a number of undesirable Fili
pinos aro being recruited and for
warded to Hawaii, s tho belief of tho
Honolulu pollco nuthorlUcs. There
Iibb been an epidemic of robberies In
which Filipinos havo boon mixed up
und Chief McDullle finds that ho has
to havo u special Filipino ofllrcr to
copo with tho difficult.
Judging from tho photographs of
criminals thnt havo been sent from
Manila to tho Sheriff hero, thoro aro
nt itrnannt n nil tit lint nf nanmiAil lull.
a um, wanle(, mM . ,ttWn Tw()
wI, ,, ,,, lir,,n . M.
IN THE ESTATE
(Continued from Page 1)
the two children, January 23, 1890.
rrom that time until 1907, tho ac
counts wero rendered ns n joint one.
mu "" 'euruary -i oi mat. year in"
Runrdlan petitioned that tho estate
bruary l:f, 1901 78.C02.0. j
rcuruiiry 11, i.mi; au.iiu.ji
February 2, 1803 89,585.13 j
March 4, 1904 93,072.39
February 11, 1905 98,873.55 j
February 13, 1900 111,941.59 !
February 21. ,1907. . . ... 122,832.13
From this Ulme onward the ac-'
counts of tho two minors were filed '
separately, th'o Ilrst ndount of f
Henry Ca.ltcrJUlIeldBoppfntcly being
on February 2l"!'l90S, 'when his es
tate was reported as amounting to
$80,221.01. During thlH year u
ktclck dividend of 14C shares or C
llrcwer &. Co.,LUl..,wn? received b
tho guardian, of the par value of
In tho account tiled March I'l.
1909. the estato had ln:reated lo
$87,591.77. and a ear later, hen
thn annual account was Tiled, on
.January 3, 1910, the total value wan
placed at $90,1.18.55.'
John Marcalllno. In Ills report us
master, states thut while tho pres
ent account shows thnt tho Inven
toried value of tho estate Is $99,
130,72, as a mutter of fact It Is
worth considerably more, ns the
Hi ewer stock Is Inventoried nt par.
Tho master calls nttonUon of tho
court to tho clear and businesslike
management of tho ('state and to
the high-class Investments whi h
wero made with tlyi money of tho
estato by tho guardian.
Attention Is also called 'to tho
education which tho guardian has
Insisted thut )oiing Curlor should
obtain so that when ho became of
ago he would be ablo to manage his
fioven largu Bteamers 1110 duo lo
arrhe ut Hon'oliilii from almost as
many different ports during thu com
ing week. I
Moudn) should see thu arrival of '
the Mutbon Navigation stunner 1 1 i lo- (
nlau villi a caigo ami a few passeu-1
gors fixmi San Francisco by tho way
Tho American-Hawaiian freighter
Mexican with freight from Now York
via Teliiinntepec. Han Diego, San
Francisco uml Soattlo should nrrlvo
hero as well as tho United States sup
ply ship mid unvul transport Uuffalo,
on the sumo dale.
Tho now Canadian-Australian llmr
Zoahindla Is duo to show up oft tho
lxirt on Tuesday afternoon. This ves
sel Is milking her. uinldcn trip from
Sydney. N. S. W , by Hid way of Suvu.
The principal movement in shipping
on WedueBilay wll bo the departure
of tho Matsoh Navigation liner Wll
helmlnn for 'Bun. 1'iunclsco.
On Thursduy tho raclllc Mall lin
er Mongollu with u largo list of pas
sengois and bdiiio' i.irgo from San
Francisco should arrlvo hero and will
probably bo dispatched for tho Orient
tho uvening of tho sumo day.
Thu Canudhin-Aiistiallan steamer
Mnkuiu fiom Vlctoiln eiuouto lo Aus
tiullun ports Ib expeeteil will cull hoio
on Filday This vessel ran bo counted
uiKui in In lug 11 number of passeu
geis Accoidiim to preaeilt calcuUtloni of
II. Hnckfold and Company tho Japan
Z Limited S
Isaijr- -n ii 11 ic--can
eso steamer Nippon Alunrirbiu "Hong
kong via Japan jsirls should arrive
at Honolulu 011 Saturday noon.
Altogether tho coming week is des
tined to bo one productlvo of much
activity In shipping circles,
THU UNITi:i) Staters army IraiiH
poit Logan has arrived at San Fran
cisco alter her voyage from Manila by
tho way of Nagasaki and Honolulu.
The Ideal system of burial. The method beintr adopted everywhere throughout the civilized
world. Approved by the Hawaiian Board of Health.
The Townsend Undertaking Co., Ltd.,
Who have added to their business a Wholesale Department of all Undertaking- Goods. Mail
and Telephone Orders premptly attended to. Send for full particulars. Below are samples of
Black Cloth Caskets, nicely trimmed, - - $40.00
With outside bGX ready for shipment or delivery
Coffins, full sized, - $10, $20, and upwards
According to finish
69 Beretania Street
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On Track, Road and Tour
No car for llic price has ever
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l'ew cars that sell for more have
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Specifications for the beautiful
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NATIONAL GUARD OF HAWAII.
Hcaihiuaiters, First Infantry.
Honolulu, August 13, 1910.
General Orders No. 11.
Companies und detachments of tho
Fiist Infantry, National Guard of
Hawaii, stationed ut Honolulu, will
assemble under arms at tho Drill -shed
011 Tuesday, August 10, at 7:30
For Full Particulars Inquiic of
83 Merchant Street
p. in., for drill und parade.
lly order of Colonel Zlcglcr.
(Signed) TIIOS, I'. CUMMINS,
Captain and Adjutant, 1st Infantry,
N. (1. I!., Adjutant.
FA en tho girl who prides herself on
her self-possession gives It up when
tho right man asks fur It.
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